Whole-cell Patch-clamp Recordings in Brain Slices

Whole-Cell Patch-Clamp Recordings

The patch-clamp recording technique, which measures ionic currents under voltage clamp, was designed to study small patches of membranes in which near perfect control of the transmembrane voltage can be achieved readily. The recent application of patch-clamp methodology to the analysis of whole-cell current actually defies many of the original design requirements. Nevertheless, whole-cell recor...

Electrophysiological and morphological characterization of neuronal microcircuits in acute brain slices using paired patch-clamp recordings.

The combination of patch clamp recordings from two (or more) synaptically coupled neurons (paired recordings) in acute brain slice preparations with simultaneous intracellular biocytin filling allows a correlated analysis of their structural and functional properties. With this method it is possible to identify and characterize both pre- and postsynaptic neurons by their morphology and electrop...

Whole Cell Patch-Clamp Recordings from Lobula plate tangential cells in drosophila melanogaster

Whole-cell patch-clamp recordings from identified spinal neurons in the zebrafish embryo.

We describe a preparation for obtaining patch-clamp recordings from identified embryonic spinal cord interneurons, motoneurons and sensory neurons in an in vivo zebrafish preparation. This preparation is used to study the spatial and temporal patterns of spontaneous and touch-evoked electrical activity during the initial development of circuitry in the spinal cord. The combination of these phys...

Characterization of synaptic components in rat hippocampal slices using whole cell patch clamp techniques.

Brain slices from many regions of the central nervous system are an often utilized experimental system for the study of synaptic physiology and pharmacology. The hippocampal slice [ I ] has been the most highly studied of such preparations, due to its anatomically distinct synaptic pathways and disposition to synaptic plasticity [2]. The patch clamp technique [3] has permitted many new insights...